Surface properties of microbial cells probed at the nanometre scale with atomic force microscopy

2000 ◽  
Vol 30 (1) ◽  
pp. 32-35 ◽  
Author(s):  
C. J. P. Boonaert ◽  
P. G. Rouxhet ◽  
Y. F. Dufr�ne
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Properties ◽  
Microbial Cells ◽  
Force Microscopy ◽  
Atomic Force

Nanoscale Mapping of the Elasticity of Microbial Cells by Atomic Force Microscopy

Langmuir ◽  
2003 ◽  
Vol 19 (11) ◽  
pp. 4539-4543 ◽  
Author(s):  
Ahmed Touhami ◽  
Bernard Nysten ◽  
Yves F. Dufrêne
Keyword(s):  
Atomic Force Microscopy ◽  
Microbial Cells ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals An investigation of surface properties, local elastic modulus and interaction with simulated pulmonary surfactant of surface modified inhalable voriconazole dry powders using atomic force microscopy

RSC Advances ◽  
2016 ◽  
Vol 6 (31) ◽  
pp. 25789-25798 ◽  
Author(s):  
Sumit Arora ◽  
Michael Kappl ◽  
Mehra Haghi ◽  
Paul M. Young ◽  
Daniela Traini ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Surface Properties ◽  
Pulmonary Surfactant ◽  
Dry Powders ◽  
Force Microscopy ◽  
Atomic Force ◽  
Surface Modified ◽  
Spray Dried

l-Leucine modified voriconazole spray dried micropartcles.

scholarly journals Atomic Force Microscopy in Microbiology: New Structural and Functional Insights into the Microbial Cell Surface

mBio ◽  
2014 ◽  
Vol 5 (4) ◽  
Author(s):  
Yves F. Dufrêne
Keyword(s):  
Atomic Force Microscopy ◽  
Cell Surface ◽  
Cell Surface Molecules ◽  
Microbial Cells ◽  
Force Microscopy ◽  
Surface Molecules ◽  
Atomic Force ◽  
Important Research Topic ◽  
Sense And Respond ◽  
Made In

ABSTRACT Microbial cells sense and respond to their environment using their surface constituents. Therefore, understanding the assembly and biophysical properties of cell surface molecules is an important research topic. With its ability to observe living microbial cells at nanometer resolution and to manipulate single-cell surface molecules, atomic force microscopy (AFM) has emerged as a powerful tool in microbiology. Here, we survey major breakthroughs made in cell surface microbiology using AFM techniques, emphasizing the most recent structural and functional insights.

Using Atomic Force Microscopy to Retrieve Nanomechanical Surface Properties of Materials

2006 ◽  
Vol 514-516 ◽  
pp. 1598-1602 ◽  
Author(s):  
Sergio Graça ◽  
Rogerio Colaço ◽  
Rui Vilar
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Properties ◽  
Application Rate ◽  
Critical Parameters ◽  
Nanomechanical Property ◽  
Force Microscopy ◽  
Atomic Force ◽  
Properties Of Materials ◽  
Microscopy Techniques ◽  
Scratch Tests

When atomic force microscopy is used to retrieve nanomechanical surface properties of materials, unsuspected measurement and instrumentation errors may occur. In this work, some error sources are investigated and operating and correction procedures are proposed in order to maximize the accuracy of the measurements. Experiments were performed on sapphire, Ni, Co and Ni-30%Co samples. A triangular pyramidal diamond tip was used to perform indentation and scratch tests, as well as for surface visualization. It was found that nonlinearities of the z-piezo scanner, in particular the creep of the z-piezo, and errors in the determination of the real dimensions of tested areas, are critical parameters to be considered. However, it was observed that there is a critical load application rate, above which the influence of the creep of the z-piezo can be neglected. Also, it was observed that deconvolution of the tip geometry from the image of the tested area is essential to obtain accurate values of the dimensions of indentations and scratches. The application of these procedures enables minimizing the errors in nanomechanical property measurements using atomic force microscopy techniques.

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